| Gene Introduced (1st): |
PsGPT |
| Gene Source (1st): |
Manihot esculenta Granule Bound Starch Synthase 1 promoter and Agrobacterium tumefaciens nopaline synthase terminator |
| Function (1st): |
Overexpression of PsGPT that codes for a membrane-bound translocator that transportd glucose-6-phosphate into the amyloplasts |
| Gene Introduced (2nd): |
AtNTT1 |
| Gene Source (2nd): |
Transcribed from solanum tuberosum soluble starch synthase 3 promoter and translated from cucumber mosaic virus 1 5 UTR |
| Function (2nd): |
Over expression of AtNTT1 that encodes for transporting ATP into amyloplasts. |
|
Starch biosynthesis is dependent on presence of both glucose-6-phosphate and ATP. Thus PSGPT and AtNTT1 together promote starch biosynthesis in the amyloplasts, leading to greater accumulation of starch in the storage roots of the cassava plants. |
| Gene Introduced (3rd): |
AtTMT1 |
| Gene Source (3rd): |
Trancribed from A. thaliana Rubsico small Subunit 3B promoter and translated from Potato Virus X 5 UTR and Agrobacterium tumefaciens Gene 7 terminator |
| Function (3rd): |
Overexpressing of AtTMT1 leading to an increased expression of photosynthetic genes, a decrease in sugar consumption for cellular respiration, a reduction of nocturnal carbondioxide loss and increased export capacity of sugars from the leaves |
| Gene Introduced (4th): |
hairpin RNA (hpRNA) |
| Gene Source (4th): |
Transcription from A. thaliana Rubisco small subunit 2B promoter and transcribes an inverted repeat of sequences complementary to AC1, before terminating at the Cauliflower Mosiac Virus 35S terminator. |
| Function (4th): |
Following processing of the hpRNA, the host RNAi proteins target viral transcripts with complementary base pairs to AC1 inverted repeats thus preventing viral replication and infection via destruction of AC1, a gene essential for viral replication |
